Application
Flow cytometric analysis of Jurkat cells, using anti-HDAC10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Anti-HDAC10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HDAC10Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-HDAC10mouse monoclonal antibody. ( Dilution 1:50)Application
Flow cytometric analysis of Hela cells, using anti-HDAC10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HDAC10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-HDAC10mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.| Product Name | HDAC10 antibody [2H6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H6] to HDAC10Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. |
| Immunogen | Full length human recombinant protein of human HDAC10 (NP_114408) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2H6 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZP761B039 |
|---|---|
| Target | HDAC10 |
| Tissue Specificity | is specific for human HDAC10 (NP_114408). |
| Cellular Localization | Excluded from the nucleoli. |
Application
Flow cytometric analysis of Jurkat cells, using anti-HDAC10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Anti-HDAC10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HDAC10
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-HDAC10mouse monoclonal antibody. ( Dilution 1:50)
Application
Flow cytometric analysis of Hela cells, using anti-HDAC10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HDAC10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-HDAC10mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
