Application
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using HDAC2 (Phospho-Ser394) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2(Phospho-Ser394) Antibody| Product Name | HDAC2 (phospho Ser394) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC2 (Phospho Ser394)Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes |
| Immunogen | Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2. |
| Modification | p-Ser394 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HD2, RPD3, YAF1, HDAC2, histone deacetylase 2, transcriptional regulator homolog RPD3, YY1-associated factor 1 |
|---|---|
| Target | HDAC2 (Phospho Ser394) |
| Molecular Weight(MW) | 60 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using HDAC2 (Phospho-Ser394) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2(Phospho-Ser394) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
