Application
Immunofluorescence staining of methanol-fixed Hela cells using HDAC2 Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 and Hela cells using HDAC2 Antibody .Application
Western blot analysis of extracts from 293 and Hela cells using HDAC2(Ab-394) Antibody| Product Name | HDAC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC2Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes |
| Immunogen | Peptide sequence around aa. 392~396 (E-D-S-G-D) derived from Human HDAC2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HD2, RPD3, YAF1, HDAC2, histone deacetylase 2, transcriptional regulator homolog RPD3, YY1-associated factor 1 |
|---|---|
| Target | HDAC2 |
| Molecular Weight(MW) | 60 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using HDAC2 Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 and Hela cells using HDAC2 Antibody .
Application
Western blot analysis of extracts from 293 and Hela cells using HDAC2(Ab-394) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
