Application
HDAC2 antibody detects HDAC2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE HDAC2 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HDAC2 antibody detects HDAC2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC2 protein stained by HDAC2 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Product Name | HDAC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC2 (histone deacetylase 2)This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HDAC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Chicken, BovineMouse (100%), Xenopus laevis (99%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HD2 antibody, RPD3 antibody, YAF1 antibody, HDAC2 antibody, histone deacetylase 2 antibody, transcriptional regulator homolog RPD3 antibody, YY1-associated factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Nucleus |
Application
HDAC2 antibody detects HDAC2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE HDAC2 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HDAC2 antibody detects HDAC2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC2 protein stained by HDAC2 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Positive Control | rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
