Application
Confocal immunofluorescence analysis of Hela cells using anti-HDAC3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.Application
Western blot analysis using anti-HDAC3 mAb against Hela (1), NIH/3T3 (2), C6 (3) and COS (4) cell lysate.| Product Name | HDAC3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7G6C5] to HDAC3Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 224 and 428 of human HDAC3 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 7G6C5 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RPD3-2 HD3 RPD3 |
|---|---|
| Target | HDAC3 |
| Molecular Weight(MW) | 48.8 kDa (note) |
Application
Confocal immunofluorescence analysis of Hela cells using anti-HDAC3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Application
Western blot analysis using anti-HDAC3 mAb against Hela (1), NIH/3T3 (2), C6 (3) and COS (4) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
