Application
ChIP was performed with HeLa nuclear extract and either 5 μg of HDAC3 antibody or control mouse IgG. The precipitated DNA was detected by QPCR with primer set targeting to p21 or GAPDH promoter.Application
WB analysis of HeLa cells using HDAC3 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500 (red). The nuclei were stained with DAPI.| Product Name | HDAC3 antibody [GT9007] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [GT9007] to HDAC3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | GT9007 |
| Host Species | Mouse |
| Tested Applications | ICC/IFChIP |
| ChIP (2 µg/ChIP) | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | RPD3, HD3, RPD3-2, histone deacetylase 3 |
|---|---|
| Target | HDAC3 |
Application
ChIP was performed with HeLa nuclear extract and either 5 μg of HDAC3 antibody or control mouse IgG. The precipitated DNA was detected by QPCR with primer set targeting to p21 or GAPDH promoter.
Application
WB analysis of HeLa cells using HDAC3 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500 (red). The nuclei were stained with DAPI.| Application Notes | ChIP (2 µg/ChIP) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
