Application
ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.Application
ChIP was performed with HeLa extracts and either 5 μg of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.| Product Name | HDAC3 antibody [GT9080] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [GT9080] to HDAC3Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | GT9080 |
| Host Species | Mouse |
| Tested Applications | ICC/IFChIP |
| ChIP (5 μl/ChIP) | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RPD3 , HD3 , RPD3-2 , histone deacetylase 3 |
|---|---|
| Target | HDAC3 |
Application
ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.
Application
ChIP was performed with HeLa extracts and either 5 μg of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.| Application Notes | ChIP (5 μl/ChIP) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Cell culture containing 0.05% azide. |
