Application
Anti-HDAC6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HDAC6Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.Application
Flow cytometric Analysis of Hela cells, using anti-HDAC6 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Flow cytometric Analysis of Jurkat cells, using anti-HDAC6 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC209649 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HDAC6 antibody, and then analyzed by flow cytometry.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.| Product Name | HDAC6 antibody [1D11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1D11] to HDAC6Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. |
| Immunogen | Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JM21, HDAC6, HD6 |
|---|---|
| Target | HDAC6 |
| Tissue Specificity | is specific for human HDAC6 (NP_006035). |
| Cellular Localization | It is mainly cytoplasmic, where it is associated with microtubules. |
Application
Anti-HDAC6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HDAC6
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.
Application
Flow cytometric Analysis of Hela cells, using anti-HDAC6 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Flow cytometric Analysis of Jurkat cells, using anti-HDAC6 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC209649 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HDAC6 antibody, and then analyzed by flow cytometry.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
