Application
WB analysis of (1) HepG2, (2) SKBR-3, (3) Jurkat and (4) Raji lysates (10 μg per lane) using HDJ2 antibody [EPR7248] at a dilution of 1:10,000.Application
FACS analysis of permeabilized Jurkat cells using HDJ2 antibody [EPR7248] at a dilution of 1:10 (red) or a rabbit IgG control (green).| Product Name | HDJ2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody to HDJ2HSP40 (heat shock protein 40) proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. It has been found that HSP40-4 is a partner DnaJ for the Hsc70-based chaperone system. HSP40-4 is know to play a role in protein import into the mitochondria. |
| Immunogen | A synthetic peptide corresponding to residues near the C-terminus of human HDJ2 was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR7248 |
| Host Species | Rabbit |
| Tested Applications | FACSIPWB |
| FACS:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | DJ-2 DjA1 HDJ2 HSDJ HSJ2 HSPF4 NEDD7 hDJ-2 |
|---|---|
| Target | HDJ2 |
Application
WB analysis of (1) HepG2, (2) SKBR-3, (3) Jurkat and (4) Raji lysates (10 μg per lane) using HDJ2 antibody [EPR7248] at a dilution of 1:10,000.
Application
FACS analysis of permeabilized Jurkat cells using HDJ2 antibody [EPR7248] at a dilution of 1:10 (red) or a rabbit IgG control (green).| Application Notes | FACS:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 49% PBS, 50% Glycerol, 0.05% BSA, pH: 7.20 and 0.01% Sodium azide |
