Application
IHC-P analysis of human colon carcinoma with (left) or without (right) Heat Shock Protein 104 antibody [Antiserum 8-1] at a dilution of 1:100. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.Application
IHC-P analysis of human liver tissue with (left) or without (right) Heat Shock Protein 104 antibody [Antiserum 8-1] at a dilution of 1:200. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.Application
IHC-P analysis of human tonsil tissue with (left) or without (right) Heat Shock Protein 104 antibody [Antiserum 8-1] at a dilution of 1:100. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.| Product Name | Heat Shock Protein 104 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Heat Shock Protein 104Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. The HSP100 proteins generally have amino acid sequences of about 900 residues and contain two nucleotide-binding sites. Within the HSP100 family of proteins, yeast express an ~104 kDa form which is necessary to protect cells from various stress conditions such as heat, heavy metals and ethanol, though mutation studies have shown that the protein is not required for normal growth. Yeast HSP104 has been shown to be a ClpB protein with significant sequence homology to E. coli ClpB, particularly in the two nucleotide-binding sites. |
| Immunogen | Synthetic peptide corresponding to C-terminal residues D(894) D D N E D S M E I D D D L D(908) of S. cerevisiae HSP104. |
| Clonality | Polyclonal |
|---|---|
| Clone Number | Antiserum 8-1 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| IHC: 1:100 - 1:200: |
|
| Species Reactivity | HumanYeast |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSP104 |
|---|---|
| Target | Heat Shock Protein 104 |
Application
IHC-P analysis of human colon carcinoma with (left) or without (right) Heat Shock Protein 104 antibody [Antiserum 8-1] at a dilution of 1:100. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Application
IHC-P analysis of human liver tissue with (left) or without (right) Heat Shock Protein 104 antibody [Antiserum 8-1] at a dilution of 1:200. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Application
IHC-P analysis of human tonsil tissue with (left) or without (right) Heat Shock Protein 104 antibody [Antiserum 8-1] at a dilution of 1:100. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.| Application Notes | IHC: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antisera containing 0.05% sodium azide. |
