| Product Name | Heavy chain cardiac Myosin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3-48] to Heavy chain cardiac Myosin3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3-48 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FWB |
| For ELISA: Use at an assay dependant dilution. For IF: Use at an assay dependant dilution. For IHC-Fr: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Predicted molecular weight: 223 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDogPigRatRabbitBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ASD3 160710 Myosin cardiac Heavy chain alphaMHC alpha-MHC 4624 MYHCA P13533 MYH6 MYHC alpha MHC |
|---|---|
| Target | Heavy chain cardiac Myosin |
| Tissue Specificity | This antibody is myosin alpha and beta heavy chain specific. Affinity constants for:Human ventricular myosin heavy chains - 3.33 x 10 8 M -1 Human atrial myosin heavy chains - 1.48 x 10 8 M -1 Human skeletal muscle myosin - 1.06 x 10 8 M -1 Canine cardiac myosin heavy chains - 2.06 x 10 8 M -1 3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells. |
| Cellular Localization | Thick filaments of the myofibrils |
| Light Chain Type | kappa |
| Positive Control | Slow skeletal muscle, fast skeletal muscle, eye muscle |
|---|---|
| Application Notes | For ELISA: Use at an assay dependant dilution. For IF: Use at an assay dependant dilution. For IHC-Fr: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Predicted molecular weight: 223 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline |
