Application
Immunofluorescent staining of Hec1 non-phospho Ser55 in cells undergoing prometaphase (top) or metaphase (bottom)| Product Name | Hec1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hec1HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM] |
| Immunogen | A Synthetic peptide within a.a. 30~80 around Ser55 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| For WB: Use at a dilution of 1:750: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEC antibody hsNDC80 antibody NDC80 homolog antibody kinetochore complex component (S. cerevisiae) antibody NDC80 antibody KNTC2 antibody TID3 antibody HEC2 antibody Hec1 (non-phospho Ser 55) antibody |
|---|---|
| Target | Hec1 |
| Molecular Weight(MW) | 80 kDa (note) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Immunofluorescent staining of Hec1 non-phospho Ser55 in cells undergoing prometaphase (top) or metaphase (bottom)| Positive Control | HeLa , Blocking peptide (GTX70016-PEP) |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:750: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1X PBS |
