Application
ELISA detection of Helicobacter pylori urease B using for capture and for detection. Coating Ab: mouse anti-ureB(, 100 ng/well ) Sample: H. pylori. Lysate (OD600 = 0.08~0.1, 5x dilute from above) Capture Ab: rabbit anti-ureB(, 1:1000) 2-Ab: goat anti-rabbit IgG-HRP (1:1000)Application
Sample (0.1 ug of whole cell lysate) A: Helicobacter pylori 7.5% SDS PAGE diluted at 1:10000| Product Name | Helicobacter pylori urease B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Urease B (Helicobacter pylori Urease B)Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT1263 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Helicobacter pylori |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Urease B Helicobacter pylori Urease B |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) |
Application
ELISA detection of Helicobacter pylori urease B using for capture and for detection. Coating Ab: mouse anti-ureB(, 100 ng/well ) Sample: H. pylori. Lysate (OD600 = 0.08~0.1, 5x dilute from above) Capture Ab: rabbit anti-ureB(, 1:1000) 2-Ab: goat anti-rabbit IgG-HRP (1:1000)
Application
Sample (0.1 ug of whole cell lysate) A: Helicobacter pylori 7.5% SDS PAGE diluted at 1:10000| Positive Control | Helicobacter pylori |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
