Application
Western blot analysis of HO-1 pAb: Lane 1: HO-1 Recombinant Protein Lane 2: Human Liver Microsomes Lane 3: Rat Liver Microsomes Lane 4: Mouse Liver Microsomes Lane 5: Canine Liver MicrosomesApplication
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.Application
Immunohistochemistry analysis of Alzheimer's diseased section stained with HO-1 (Hsp32) pAb.| Product Name | Heme Oxygenase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Heme Oxygenase 1Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant. |
| Immunogen | Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500~1:2000 IHC:1:50~1:200 |
|
| Species Reactivity | DogGuinea PigHamsterHumanMonkeyMousePigRabbitRatSheep |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Heme Oxygenase 1 Ho1 Heox 141250 hsp32 3162 Heme Oxygenase1 P09601 Hmox1 Hmox HO-1 bK286B10 HEOXG HO 1 |
|---|---|
| Target | Heme Oxygenase 1 |
| Molecular Weight(MW) | 34.6 kDa |
| Tissue Specificity | May cross-react with HO-2 in western blot. It also recognizes a 50kD band found only in transformed cell lines but not in normal tissues. |
Application
Western blot analysis of HO-1 pAb: Lane 1: HO-1 Recombinant Protein Lane 2: Human Liver Microsomes Lane 3: Rat Liver Microsomes Lane 4: Mouse Liver Microsomes Lane 5: Canine Liver Microsomes
Application
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.
Application
Immunohistochemistry analysis of Alzheimer's diseased section stained with HO-1 (Hsp32) pAb.| Positive Control | Recombinant Human and Rat HO-1 (Hsp32) Protein |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 50% glycerol and 0.09% sodium azide |
