Application
Western blot analysis of HO-2 expression with rat kidney tissue lysate.| Product Name | Heme Oxygenase-2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Heme Oxygenase-2Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin. Two heme oxygenase isozymes, termed HO-1 and HO-2, have been identified so far. At the amino acid level, HO-1 and HO-2 are 42% homologous. However, each isozyme is evolutionary highly conserved. HO-1 is the inducible form of the enzyme and is therefore also referred to as stress protein Hsp32. Expression of HO-1 can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is the constituitive form of the enzyme. It is not inducible by exogeneous stimuli and HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant under normal conditions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIPWB |
| Western blotting (0.5-4 µg/ml), in immunoprecipitation (20 µg/ml) and Immunohistochemistry (20 µg/ml, frozen section tested). However, the optimal concentrations should be determined individually. The antibody recognizes ~36 kDa Heme-Oxygenase-2 of human, mouse, rat, hamster, and monkey origins.: |
|
| Species Reactivity | HumanMouseMonkeyRatHamster |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EC1.14.99.3 HO2 HO-2 EC 1.14.99.3 HMOX2 Heme Oxygenase2 HO 2 |
|---|---|
| Target | Heme Oxygenase-2 |
| Tissue Specificity | This antibody is specific for Heme Oxygenase-2. |
Application
Western blot analysis of HO-2 expression with rat kidney tissue lysate.| Application Notes | Western blotting (0.5-4 µg/ml), in immunoprecipitation (20 µg/ml) and Immunohistochemistry (20 µg/ml, frozen section tested). However, the optimal concentrations should be determined individually. The antibody recognizes ~36 kDa Heme-Oxygenase-2 of human, mouse, rat, hamster, and monkey origins.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
