Application
Hemoglobin zeta antibody [GT1544] detects Hemoglobin zeta protein at cytoplasm by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hemoglobin zeta protein stained by Hemoglobin zeta antibody [GT1544] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin zeta antibody [GT1544] diluted at 1:1000.| Product Name | Hemoglobin zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1544] to Hemoglobin zeta (hemoglobin, zeta)Zeta-globin is an alpha-like hemoglobin. The zeta-globin polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult like. The zeta-globin gene is a member of the human alpha-globin gene cluster that includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Hemoglobin zeta. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT1544 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBZ antibody Hemoglobin zeta antibody [GT1544] antibody Hemoglobin zeta antibody hemoglobin subunit zeta antibody |
|---|---|
| Target | Hemoglobin zeta |
| Molecular Weight(MW) | 16 kDa (note) |
Application
Hemoglobin zeta antibody [GT1544] detects Hemoglobin zeta protein at cytoplasm by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hemoglobin zeta protein stained by Hemoglobin zeta antibody [GT1544] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin zeta antibody [GT1544] diluted at 1:1000.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
