| Product Name | Hepatitis B surface antigen antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BID40] to Hepatitis B Virus Surface Antigen (Ad/Ay)Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. |
| Immunogen | Hepatitis B Surface antigen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | BID40 |
| Host Species | Mouse |
| Tested Applications | ELISARIALFIA |
| IA: Use at an assay dependent dilution. Pairs with Goat Anti-HBsAg Antibody (affinity purified) or with HBsAg Monoclonal antibody in sandwich immunoassay. Enzyme conjugation: Use at an assay dependent dilution. Radio labeling: Use at an assay dependent dilution. Solid phase coating: Use at an assay dependent dilution. This antibody is not for neutralization. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Hepatitis B virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBsAg |
|---|---|
| Target | Hepatitis B surface antigen |
| Tissue Specificity | Reacts with the ad and ay subtype of the Hepatitis B virus surface antigen. |
| Cellular Localization | Secreted |
| Application Notes | IA: Use at an assay dependent dilution. Pairs with Goat Anti-HBsAg Antibody (affinity purified) or with HBsAg Monoclonal antibody in sandwich immunoassay. Enzyme conjugation: Use at an assay dependent dilution. Radio labeling: Use at an assay dependent dilution. Solid phase coating: Use at an assay dependent dilution. This antibody is not for neutralization. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: 0.85% Sodium chloride, 0.015M Potassium phosphate. pH 7.4. |
