Application
Non-transfected (–) and transfected (+) Huh7 whole cell extracts (25 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis B Virus X Protein antibody [X36C] diluted at 1:1000.| Product Name | Hepatitis B Virus X Protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Hepatitis B Virus X ProteinThe hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries. Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens. |
| Immunogen | Baculovirus expressed recombinant HBx. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | X36C |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| Recommended Starting Dilutions: For ELISA: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Hepatitis B virus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HBVgp3 Hepatitis B Virus X antigen |
|---|---|
| Target | Hepatitis B Virus X Protein |
Application
Non-transfected (–) and transfected (+) Huh7 whole cell extracts (25 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis B Virus X Protein antibody [X36C] diluted at 1:1000.| Positive Control | HBV infected Huh-7 |
|---|---|
| Application Notes | Recommended Starting Dilutions: For ELISA: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. Optimal dilution / concentration should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
