Mouse monoclonal [A1/3D1] to Hepatitis C Core AntigenHepatitis C virus (HCV) is the primary cause of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. The 21 kDa core protein of HCV is well conserved among different HCV genotypes and may suppress hepatitis B virus replication in a phosphorylation dependent manner.
Immunogen
Synthetic peptides derived from HCV capsid protein.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A1/3D1
Host Species
Mouse
Tested Applications
ELISA
ELISA: Use at an assay dependent dilution. GTX18700 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), capsid protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsid protein (OD492 value > 2.0) and mixture of NS and C (OD492 value >1.5), and have no reaction with HCV non-structural protein (OD492 value:
Species Reactivity
Hepatitis C Virus
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Protein G
Target Information
Target
Hepatitis C core antigen
Tissue Specificity
detects recombinant capsid protein C (CPC), envelope protein M (EPM) core; amino acids 1-142) and synthetic capsid protein C (amino acids 1-61). recognizes different antigenic determinants of Hepatitis C Core Antigen.
Light Chain Type
kappa
Application Notes
ELISA: Use at an assay dependent dilution. GTX18700 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), capsid protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsid protein (OD492 value > 2.0) and mixture of NS and C (OD492 value >1.5), and have no reaction with HCV non-structural protein (OD492 value:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.