| Product Name | Hepatitis C virus Core protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B2] to Hepatitis C Core AntigenHepatitis C virus (HCV) is the primary cause of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. The 21 kDa core protein of HCV is well conserved among different HCV genotypes and may suppress hepatitis B virus replication in a phosphorylation dependent manner. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | B2 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. Detects human Hepatitis C Virus core protein but not HCV non-structural protein. WB: Use at a concentration of 0.1 - 0.5 μg/ml. This allows visualization of 0.1 ug/lane of recombinant CPC+EPM (core), 0.5 ug/lane synthetic CPC, and 0.1 ug/lane recombinant chimeric HCV polyprotein. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Hepatitis C Virus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Target | Hepatitis C core antigen |
|---|---|
| Tissue Specificity | detects recombinant capsid protein C and envelope protein M (1-142 aa), synthetic CPC (1-61 aa) and recombinant chimeric HCV polyprotein (60kDa). No cross reactivity is observed with recombinant NS3 protein (125-147 aa), synthetic NS3 protein (1378-1458 aa) and synthetic NS4a protein (1689-1735 aa). recognizes different antigenic determinants of HCV capsid protein. can be used to detect in-vitro translated HCV core protein. |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at an assay dependent dilution. Detects human Hepatitis C Virus core protein but not HCV non-structural protein. WB: Use at a concentration of 0.1 - 0.5 μg/ml. This allows visualization of 0.1 ug/lane of recombinant CPC+EPM (core), 0.5 ug/lane synthetic CPC, and 0.1 ug/lane recombinant chimeric HCV polyprotein. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 0.01M PBS. pH 7.0 |
