| Product Name | Hepatitis C virus NS3 protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [281] to HCV NS3HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. |
| Immunogen | Purified recombinant chimeric HCV polyprotein (555 amino acids). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 281 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 1 μg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user. GTX18664 will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 Protein. No reaction can be seen with synthetic NS-3.: |
|
| Species Reactivity | Hepatitis C Virus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Target | HCV NS3 |
|---|---|
| Tissue Specificity | reacts with recombinant NS-3 (amino acids 1252-1477), synthetic NS-3 (amino acids 1378-1458), and secombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), and synthetic NS-4a protein (amino acids 1689-1735). |
| Cellular Localization | Cell Membrane, Cytoplasmic and Plasma membrane |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 1 μg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user. GTX18664 will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 Protein. No reaction can be seen with synthetic NS-3.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 0.01M PBS. pH 7.2 |
