Application
Application| Product Name | Hepatitis C virus NS4A protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [497] to Hepatitis C Virus NS4HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. |
| Immunogen | Recombinant full length protein (Purified recombinant Chimeric HCV Polyprotein, 555aa) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 497 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 µg/ml. Detects Recombinant ChimericHCV Polyprotein at a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Hepatitis C Virus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Hepatitis C NS4 |
|---|---|
| Tissue Specificity | reacts with synthetic NS4a Protein (1689a.a.-1735a.a.) and recombinant Chimeric HCV Polyprotein (60 kDa). No cross reaction with synthetic recombinant Capsid Protein C + Envelope Protein M (core)(1a.a.-142a.a.), synthetic Capsid Protein C (1a.a.-61a.a.), recombinant NS3 Protein (1252a.a.-1477a.a.) and synthetic NS3 Protein (1378a.a.-1458a.a.) |
| Cellular Localization | RNA virus |
| Light Chain Type | kappa |
Application
Application| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 µg/ml. Detects Recombinant ChimericHCV Polyprotein at a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 0.01M PBS. pH 7.0 |
