Monoclonal Mouse Antibody to Hepatocyte Specific AntigenHepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes. This antibody recognizes an uncharacterized antigen present in both adults and fetal normal hepatocytes to produce a distinct granular cytoplasmic staining. This antibody stains majority of hepatocellular carcinomas.
Immunogen
Human liver fixed in formalin
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OCH1E5
Host Species
Mouse
Tested Applications
FACSIHC-P
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed tissues do not require any special treatment.:
Species Reactivity
HumanRat
Concentration
1mg/ml
Target Information
Target
Hepatocyte Specific Antigen
Cellular Localization
Cytoplasmic
Application
Application
Formalin fixed paraffin embedded liver stained with Hepatocyte Specific Antigen antibody .
Positive Control
Liver
Application Notes
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed tissues do not require any special treatment.: