Application
at a 1:200 dilution stainingSK-BR-3 whole cell lysate| Product Name | Her2 / ErbB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ErbB2ErbB2 (also known as Her2 or neu) is a 185 kDa transmembrane receptor tyrosine kinase from the EGFR family that acts to regulate a variety of biological responses including cell growth, differentiation, and tissue development. Ligand binding to the extracellular domain, or overexpression of the receptor leads to phosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Overexpression or abnormal activation of ErbB2 has been found in a variety of tumors including brain, breast, lung and skin cancer. Autophosphorylation of tyrosine 1139 on ErbB2 allows binding of Grb2 and the Src SH2 domain, which allows activation of the Ras, Raf, ERK1&2 and JAK/STAT signaling pathways, respectively. |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PIPWB |
| For Western Blotting, use at a dilution of 1:200 Optimal dilutions should be determined by the user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | c-erb B2 Herstatin HER2 137215 cerb B2 ErbB2 NGL HER2/neu P04626 2064 NEU HER-2/neu HER-2 TKR1 HER 2 c erb B2 HER 2/neu |
|---|---|
| Target | ErbB2 |
| Cellular Localization | Cell Membrane |
Application
at a 1:200 dilution stainingSK-BR-3 whole cell lysate| Positive Control | SK-BR-3 lysate. |
|---|---|
| Application Notes | For Western Blotting, use at a dilution of 1:200 Optimal dilutions should be determined by the user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store this product at 4 °C, do not freeze. The product is stable for one year from the date of shipment. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
