| Product Name | Her2 / ErbB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ErbB2ErbB2 (also known as Her2 or neu) is a 185 kDa transmembrane receptor tyrosine kinase from the EGFR family that acts to regulate a variety of biological responses including cell growth, differentiation, and tissue development. Ligand binding to the extracellular domain, or overexpression of the receptor leads to phosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Overexpression or abnormal activation of ErbB2 has been found in a variety of tumors including brain, breast, lung and skin cancer. Autophosphorylation of tyrosine 1139 on ErbB2 allows binding of Grb2 and the Src SH2 domain, which allows activation of the Ras, Raf, ERK1&2 and JAK/STAT signaling pathways, respectively. |
| Immunogen | Synthetic peptide: LARLLDIDETEYHAD, corresponding to amino acids 866-880 of Human ErbB2 kinase domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| For IF: Use at a concentration of 1-5 μg/ml.For IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 1-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Synonyms | c-erb B2 Herstatin HER2 137215 cerb B2 ErbB2 NGL HER2/neu P04626 2064 NEU HER-2/neu HER-2 TKR1 HER 2 c erb B2 HER 2/neu |
|---|---|
| Target | ErbB2 |
| Cellular Localization | Cell Membrane |
| Positive Control | SK-BR-3 cells |
|---|---|
| Application Notes | For IF: Use at a concentration of 1-5 μg/ml.For IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 1-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 50 mM sodium phosphate buffer, 50% glycerol containing no preservatives |
