Application
Flow cytometric analysis of 293 cells using ErbB3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ErbB3 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = hepatocarcinoma.Application
Western blot analysis of anti-ErbB3 Pab in mouse brain lysate. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | Her3 / ErbB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ErbB 3Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 41-56 of Human Erb B3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For ELISA: Use at a dilution of 1:900 - 1:1,100. For IHC-F and IHC-P: Use at a dilution of 1:50 - 1:100. For WB: Use at a dilution of 1:100 - 1:500. Detects a band of approximately 148 kDa (predicted molecular weight: 161 kDa). Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | c-erbB-3 c-erbB3 p45-sErbB3 p85-sErbB3 ErbB-3 MDABF1 ErbB3 P21860 erbB3-S p180ErbB3 p85sErbB3 erbB3S cerbB3 p45sErbB3 190151 MGC88033 2065 HER3 MDA-BF-1 p180-ErbB3 ErbB 3 c erbB3 erbB3 S c erbB 3 MDA BF 1 p45 sErbB3 p85 sErbB3 p |
|---|---|
| Target | ErbB3 |
| Cellular Localization | Type I membrane protein and Secreted |
Application
Flow cytometric analysis of 293 cells using ErbB3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ErbB3 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = hepatocarcinoma.
Application
Western blot analysis of anti-ErbB3 Pab in mouse brain lysate. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Positive Control | Mouse brain lysate. |
|---|---|
| Application Notes | For ELISA: Use at a dilution of 1:900 - 1:1,100. For IHC-F and IHC-P: Use at a dilution of 1:50 - 1:100. For WB: Use at a dilution of 1:100 - 1:500. Detects a band of approximately 148 kDa (predicted molecular weight: 161 kDa). Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 0.09% sodium azide |
