| Product Name | Her3 / ErbB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [RTJ1] to ErbB 3This antibody reacts with normal gastrointestinal epithelium as well as gastrointestinal and breast tumors, resulting in a cytoplasmic staining pattern. |
| Immunogen | Synthetic peptide corresponding to a site on the cytoplasmic domain of the human c-erbB-3 oncoprotein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | RTJ1 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-PWB |
| This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. In a standard three-step streptavidin-biotin method a dilution of 1:40 may be used as a guideline. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | c-erbB-3 c-erbB3 p45-sErbB3 p85-sErbB3 ErbB-3 MDABF1 ErbB3 P21860 erbB3-S p180ErbB3 p85sErbB3 erbB3S cerbB3 p45sErbB3 190151 MGC88033 2065 HER3 MDA-BF-1 p180-ErbB3 ErbB 3 c erbB3 erbB3 S c erbB 3 MDA BF 1 p45 sErbB3 p85 sErbB3 p |
|---|---|
| Target | ErbB3 |
| Tissue Specificity | This antibody is specific against c-erbB-3 oncoprotein that is expressed in a variety of human normal, fetal and neoplastic epithelial tissues. |
| Cellular Localization | Type I membrane protein and Secreted |
| Light Chain Type | kappa |
| Application Notes | This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. In a standard three-step streptavidin-biotin method a dilution of 1:40 may be used as a guideline. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 10mM PBS, pH 7.4, with 0.2% BSA and |
