Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with HERC5 antibody diluted at 1:1000.| Product Name | HERC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HERC5 (HECT and RLD domain containing E3 ubiquitin protein ligase 5)This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HERC5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CEBP1 HECT and RLD domain containing E3 ubiquitin protein ligase 5 CEB1 |
|---|---|
| Target | HERC5 |
| Molecular Weight(MW) | 117 kDa (note) |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with HERC5 antibody diluted at 1:1000.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
