Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAD23B antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat C: Raji 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAD23B antibody at 1:500 dilution.| Product Name | hHR23b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hHR23b (RAD23 homolog B (S. cerevisiae))The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RAD23B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, Chimpanzee, BovineMouse (96%), Rat (97%), Xenopus laevis (80%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HHR23B antibody HR23B antibody P58 antibody RAD23B antibody XP-C repair complementing protein antibody XP-C repair complementing complex 58 kDa antibody UV excision repair protein RAD23 homolog B antibody XP-C repair-complementing complex 58 kDa pro |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (4) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAD23B antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat C: Raji 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAD23B antibody at 1:500 dilution.| Positive Control | A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
