Application
Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [ - Detection of HIF-1 Alpha (green) in RCC4 cells using Nuclei (Blue) were counterstained using Hoechst 33258.Application
Immunohistochemistry: HIF-1 alpha Antibody [ - Staining of placenta, villi.Application
Immunohistochemistry: HIF-1 alpha Antibody [ - Analysis of HIF-1a in normal mouse kidneyApplication
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ - Analysis of HIF-1a in human kidney tumorApplication
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ - Analysis of HIF-1a in paraffin section of mouse skin wound using at 1:6,000.Application
Western Blot: HIF-1 alpha Antibody [ - Detection of HIF-1 alpha in a hypoxic sample Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.Application
Western Blot: HIF-1 alpha Antibody [ - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.| Product Name | HIF1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to mouse HIF 1 alphaHypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets. |
| Immunogen | A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PIPWBChIP |
| For WB: Use at a dilution of 1:500-1:2000: |
|
| Species Reactivity | HumanMouseRatGoatHamsterPrimateRabbit |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | AA959795 HIF1 alpha HIF-1alpha MOP1 Hif1a 15251 HIF1alpha HIF 1alpha |
|---|---|
| Target | HIF1 alpha |
| Tissue Specificity | This antibody is specific for mouse HIF-1 alpha. |
Application
Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [ - Detection of HIF-1 Alpha (green) in RCC4 cells using Nuclei (Blue) were counterstained using Hoechst 33258.
Application
Immunohistochemistry: HIF-1 alpha Antibody [ - Staining of placenta, villi.
Application
Immunohistochemistry: HIF-1 alpha Antibody [ - Analysis of HIF-1a in normal mouse kidney
Application
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ - Analysis of HIF-1a in human kidney tumor
Application
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ - Analysis of HIF-1a in paraffin section of mouse skin wound using at 1:6,000.
Application
Western Blot: HIF-1 alpha Antibody [ - Detection of HIF-1 alpha in a hypoxic sample Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.
Application
Western Blot: HIF-1 alpha Antibody [ - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.| Application Notes | For WB: Use at a dilution of 1:500-1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 0.1 ml of protein affinity purified antisera containing 0.05% sodium azide. |
