Application
HIP55 antibody [N1], N-term detects DBNL protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DBNL protein stained by HIP55 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HIP55 antibody at 1:100 dilution.Application
Sample(30 μg of whole cell lysate) A:HeLa S3 B:MOLT4 10% SDS PAGE diluted at 1:1500| Product Name | HIP55 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIP55 (drebrin-like)Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP55. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (92%), Rat (92%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ABP1 antibody HIP-55 antibody SH3P7 antibody DBNL antibody SH3 domain-containing protein 7 antibody drebrin-like protein antibody cervical SH3P7 antibody cervical mucin-associated protein antibody src homology 3 domain-containing protein HIP-55 ant |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (3) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell projection , lamellipodium |
Application
HIP55 antibody [N1], N-term detects DBNL protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DBNL protein stained by HIP55 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HIP55 antibody at 1:100 dilution.
Application
Sample(30 μg of whole cell lysate) A:HeLa S3 B:MOLT4 10% SDS PAGE diluted at 1:1500| Positive Control | HeLaS3 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
