Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000Application
HIP55 antibody [N1N3] detects HIP55 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HIP55 antibody [N1N3] dilution: 1:200.| Product Name | HIP55 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIP55 (drebrin-like)Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIP55. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (95%), Rat (96%), Xenopus laevis (86%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABP1 antibody HIP-55 antibody SH3P7 antibody DBNL antibody SH3 domain-containing protein 7 antibody drebrin-like protein antibody cervical SH3P7 antibody cervical mucin-associated protein antibody src homology 3 domain-containing protein HIP-55 ant |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000
Application
HIP55 antibody [N1N3] detects HIP55 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HIP55 antibody [N1N3] dilution: 1:200.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
