| Product Name | Histone H1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [0.T.60] to Histone H1 - ChIP GradeAlthough histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature. |
| Immunogen | Nuclei from myeloid leukemia biopsy cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 0.T.60 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| For ELISA: Use at an assay dependent dilution. For ICC: Use at a concentration of 5-20 μg/ml. For WB: Use at a concentration of 0.5-2.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | 608101 Histone H1 P60008 HILS1 373861 |
|---|---|
| Target | Histone H1 |
| Tissue Specificity | Recognizes and is specific for histone H. Doublet detected due to hyper-phosphorylation of histone H1 subpopulation. |
| Cellular Localization | Nuclear |
| Application Notes | For ELISA: Use at an assay dependent dilution. For ICC: Use at a concentration of 5-20 μg/ml. For WB: Use at a concentration of 0.5-2.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide and 40% glycerol |
