Application
Histone H1.1 antibody detects Histone H1.1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H1.1 antibody diluted by 1:1000.| Product Name | Histone H1.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H1.1 (histone cluster 1, H1a)Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Histone H1.1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H1F1 H1A H1.1 histone cluster 1 H1a HIST1 HIST1H1A |
|---|---|
| Target | Histone H1.1 |
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Histone H1.1 antibody detects Histone H1.1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H1.1 antibody diluted by 1:1000.| Positive Control | Jurkat , Jurkat NE |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
