Application
Histone H1.3 antibody detects Histone H1.3 protein at nucleus on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Histone H1.3 antibody dilution: 1:500.Application
Histone H1.3 antibody detects Histone H1.3 protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H1.3 antibody dilution: 1:500.Application
Histone H1.3 antibody detects Histone H1.3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H1.3 protein stained by Histone H1.3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Product Name | Histone H1.3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H1.3 (histone cluster 1, H1d)Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Histone H1.3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIST1H1D H1D H1F3 H1.3 histone cluster 1 H1d H1s-2 |
|---|---|
| Target | Histone H1.3 |
| Molecular Weight(MW) | 22 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H1.3 antibody detects Histone H1.3 protein at nucleus on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Histone H1.3 antibody dilution: 1:500.
Application
Histone H1.3 antibody detects Histone H1.3 protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H1.3 antibody dilution: 1:500.
Application
Histone H1.3 antibody detects Histone H1.3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H1.3 protein stained by Histone H1.3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
