Application
Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000 J/m2 UVC treatment for ) whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody at a dilution of 1:1000.Application
Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000J/m2 UVC treatment) 3dpf zebrafish embryos whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody at a dilution of 1:1000.Application
H2afx antibody detects H2afx protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. H2afx antibody dilution: 1:200.| Product Name | Histone H2A.X antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H2A.X (H2A histone family, member X)Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Histone H2A.X. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-W |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2A.X antibody histone H2A.x antibody H2a/x antibody MGC56329 antibody zgc:56329 antibody h2afx antibody H2A histone family member X antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000 J/m2 UVC treatment for ) whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody at a dilution of 1:1000.
Application
Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000J/m2 UVC treatment) 3dpf zebrafish embryos whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody at a dilution of 1:1000.
Application
H2afx antibody detects H2afx protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. H2afx antibody dilution: 1:200.| Positive Control | 3dpf zebrafish embryos (untreated) , 3dpf zebrafish embryos (treated w/wo UV irradiation) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
