Application
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Histone H2A.XS139ph (phospho Ser139) antibody diluted at 1:250.Application
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 30 µM cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated for 2hr) B. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr) C. 30 µg HCT116 whole cell lysate/extract (untreated for 6hr) D. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10uM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.X (phospho Ser139) antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]Application
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 µg of whole cell lysate) A: HCT116 control 0 hr B: HCT116 treated with 30 µM cisplatin for 24 hr 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated) B. 30 µg NIH-3T3 whole cell lysate/extract (30µM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Histone H2A.XS139ph (phospho Ser139) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H2A.X (phospho Ser139) (H2A histone family, member X)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq] |
| Modification | p-Ser139 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | gamma H2AX H2A.XS139ph Phospho Histone H2A.X (pS139 ) |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Histone H2A.XS139ph (phospho Ser139) antibody diluted at 1:250.
Application
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 30 µM cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated for 2hr) B. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr) C. 30 µg HCT116 whole cell lysate/extract (untreated for 6hr) D. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10uM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.X (phospho Ser139) antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Application
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 µg of whole cell lysate) A: HCT116 control 0 hr B: HCT116 treated with 30 µM cisplatin for 24 hr 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated) B. 30 µg NIH-3T3 whole cell lysate/extract (30µM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HCT116 treated 30uM cisplatin 24hr , NIH-3T3 treated 30uM cisplatin 24hr , HCT116 UVB-treated 50mJ for 2hr , HCT116 UVB-treated 50mJ for 6hr , PC-12 (30uM cisplatin treatment for 24hr) , *M10 cells Adriamycin-treated |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
