Application
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis. Sample: 10uM Cisplatin for 24 hrs HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein by Western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24hr) 15 % SDS-PAGE Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] dilution: 1:1000| Product Name | Histone H2A.XS139ph (phospho Ser139) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Histone H2A.X (phospho S139) (H2A histone family, member X)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq] |
| Modification | p-Ser139 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT1021 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | gamma H2AX H2A.XS139ph Phospho Histone H2A.X (pS139 ) |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis. Sample: 10uM Cisplatin for 24 hrs HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein by Western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24hr) 15 % SDS-PAGE Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] dilution: 1:1000| Positive Control | HCT116 (30 µM Cisplatin treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
