Application
Western blot detection of phosphorylated H2AX in gamma irradiated HeLA cell lysate. Lane 1: Unphosphorylated H2AX control. Lane 2: Phosphorylated H2AXApplication
Immunofluorescent analysis of phosphorylated H2AX in control or G2/M arrested cells.| Product Name | Histone H2A.XS139ph (phospho Ser139) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3F2] to Ser139-phosphorylated H2AXHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide sequence surrounding phosphorylated Ser139. (Some references call Ser139, by the name Ser140) |
| Modification | p-Ser139 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ICC/IF: 2-4 ug/ml: |
|
| Species Reactivity | HumanMouseBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | gamma H2AX H2A.XS139ph Phospho Histone H2A.X (pS139 ) |
|---|---|
| Target | Phospho-H2AX pSer139 |
Application
Western blot detection of phosphorylated H2AX in gamma irradiated HeLA cell lysate. Lane 1: Unphosphorylated H2AX control. Lane 2: Phosphorylated H2AX
Application
Immunofluorescent analysis of phosphorylated H2AX in control or G2/M arrested cells.| Application Notes | ICC/IF: 2-4 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide and 1 mg/ml BSA |
