Application
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:200.Application
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:200.Application
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:300. Scale bar = 10 μm.Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:1000.| Product Name | Histone H2AK5ac (acetyl Lys5) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1262] to Histone H2AK5ac (acetyl Lys5) (histone cluster 2, H2aa3)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2AK5ac (acetyl Lys5). The exact sequence is proprietary. |
| Modification | acetyl Lys5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | GT1262 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Histone H2A |
|---|---|
| Molecular Weight(MW) | 14 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:200.
Application
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:200.
Application
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:300. Scale bar = 10 μm.
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2AK5ac (acetyl Lys5) antibody [GT1262] diluted at 1:1000.| Positive Control | HeLa , HeLa (0.4 μM Trichoststin A for 18 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
