Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Product Name | Histone H3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3/A antibody H3FA antibody HIST1H3A antibody histone cluster 1 H3a antibody |
|---|---|
| Target | Histone H3 |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
