Application
Histone H3.3 antibody detects H3F3A protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extrac C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg HL-60 whole cell lysate/extract F. 30 µg NCI-H929 whole cell lysate/extract 15% SDS-PAGE Histone H3.3 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3.3 antibody detects H3F3A protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract 15% SDS-PAGE Histone H3.3 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3.3 antibody detects H3F3A protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 15% SDS-PAGE Histone H3.3 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3.3 antibody detects Histone H3.3 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody dilution: 1:500.| Product Name | Histone H3.3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3.3 (H3 histone, family 3A)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq] |
| Immunogen | Full length human Histone H3.3 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRatRice |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Rabbit, BovineZebrafish (99%), Xenopus laevis (100%), Pig (100%), Rabbit (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3.3A antibody H3F3 antibody H3F3B antibody MGC87782 antibody MGC87783 antibody H3F3A antibody histone H3.3 antibody H3 histone family 3A antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3.3 antibody detects H3F3A protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extrac C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg HL-60 whole cell lysate/extract F. 30 µg NCI-H929 whole cell lysate/extract 15% SDS-PAGE Histone H3.3 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3.3 antibody detects H3F3A protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract 15% SDS-PAGE Histone H3.3 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3.3 antibody detects H3F3A protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 15% SDS-PAGE Histone H3.3 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3.3 antibody detects Histone H3.3 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody dilution: 1:500.| Positive Control | Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , *rice leaf |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
