Application
Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] Antibody: Immunoperoxidase staining of Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.Application
Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] Antibody: Immunoperoxidase staining of Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] in formalin fixed, paraffin-embedded rat duodenum at 0.2 μg/mL.Application
Whole cell extracts from serum and sodium butyrate-treated NIH3T3 cells were separated on SDS-PAGE, blotted with Anti-Acetyl- & Phospho-Histone H3 [Ac-Lys9, pSer10] and developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and substrate. Inhibition with [Ac-Lys9, pSer10] histone H3 peptide (Lane 4), no inhibition with non-acetyl-non-phospho histone H3 peptide (Lane 3), no inhibition with [Ac-Lys9] histone H3 peptide (Lane 4), and no inhibition with [pSer10] histone H3 peptide (Lane 5). Lane 1: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] Lane 2: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [Ac-Lys9, pSer10] histone H3 peptide Lane 3: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [non-Ac, non-phos] histone H3 peptide Lane 4: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [Ac-Lys9] histone H3 peptide Lane 5: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [pSer10] histone H3 peptide.Application
Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] Antibody: Immunoperoxidase staining of Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] in formalin fixed, paraffin-embedded mouse testis at 0.4 μg/mL.| Product Name | Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Histone H3 ( phospho S10 acetyl K9 )Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq] |
| Immunogen | A synthetic, acetylated and phosphorylated (acetyl K9) & (phospho S10) histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3 conjugated to KLH |
| Modification | phospho Ser10, acetyl Lys9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Detects a band of approximately 17 kDa. Optimal dilutions/concentrations should be determined by the end user. We advise you to centrifuge this product vial before use.: |
|
| Species Reactivity | HumanMouseRatXenopusBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | 601128 P84243 Q66I33 3021 Histone H3 MGC87783 H3.3A H3F3 601058 H3F3A MGC87782 H3.3B 3020 H3F3B |
|---|---|
| Target | Histone H3 |
| Tissue Specificity | Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide. |
Application
Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] Antibody: Immunoperoxidase staining of Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.
Application
Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] Antibody: Immunoperoxidase staining of Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] in formalin fixed, paraffin-embedded rat duodenum at 0.2 μg/mL.
Application
Whole cell extracts from serum and sodium butyrate-treated NIH3T3 cells were separated on SDS-PAGE, blotted with Anti-Acetyl- & Phospho-Histone H3 [Ac-Lys9, pSer10] and developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and substrate. Inhibition with [Ac-Lys9, pSer10] histone H3 peptide (Lane 4), no inhibition with non-acetyl-non-phospho histone H3 peptide (Lane 3), no inhibition with [Ac-Lys9] histone H3 peptide (Lane 4), and no inhibition with [pSer10] histone H3 peptide (Lane 5). Lane 1: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] Lane 2: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [Ac-Lys9, pSer10] histone H3 peptide Lane 3: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [non-Ac, non-phos] histone H3 peptide Lane 4: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [Ac-Lys9] histone H3 peptide Lane 5: Anti-Acetyl- & Phospho Histone H3 [Ac-Lys9, pSer10] + [pSer10] histone H3 peptide.
Application
Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] Antibody: Immunoperoxidase staining of Anti-Acetyl-& Phospho-Histone H3 [Ac-Lys9, pSer10] in formalin fixed, paraffin-embedded mouse testis at 0.4 μg/mL.| Application Notes | IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Detects a band of approximately 17 kDa. Optimal dilutions/concentrations should be determined by the end user. We advise you to centrifuge this product vial before use.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS |
