Application
Detection of Histone H3 phosphorylation in Jurkat cell lysate with Lane 1: untreated cell lysate; lane 2: Anti-Fas antibody treated Jurkat cell lysate.| Product Name | Histone H3S28ph (phospho Ser28) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [117C826] to Histone H3 ( phospho S28 )Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. |
| Immunogen | Synthetic peptide containing phosphorylated serine at position 28 (Human). |
| Modification | p-Ser28 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 117C826 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16.3 kDa). Optimal dilutions/concentrations should be determined by the end user. We advise you to centrifuge this product vial before use.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 601128 P84243 Q66I33 3021 Histone H3 MGC87783 H3.3A H3F3 601058 H3F3A MGC87782 H3.3B 3020 H3F3B Histone H3 (phospho S28) Histone H3 (phospho Ser28) Histone H3 (pS28) Histone H3 (pSer28) Histone H3 phospho S28 Histone H3 phospho Ser28 Ph |
|---|---|
| Target | Histone H3 |
| Cellular Localization | Nuclear |
Application
Detection of Histone H3 phosphorylation in Jurkat cell lysate with Lane 1: untreated cell lysate; lane 2: Anti-Fas antibody treated Jurkat cell lysate.| Positive Control | Anti-Fas mAb treated Jurkat cell lysates |
|---|---|
| Application Notes | WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16.3 kDa). Optimal dilutions/concentrations should be determined by the end user. We advise you to centrifuge this product vial before use.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
