Application
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.Application
Histone H3 (tri-Methyl Lys9) antibody detects Histone H3 (tri-Methyl Lys9) protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys9) antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 μg of Histone H3 (tri-Methyl Lys9) . Western blot analysis was performed using Histone H3 (tri-Methyl Lys9) . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.Application
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.| Product Name | Histone H3K9me3 (trimethyl Lys9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (tri-Methyl K9) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | trimethyl Lys9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3/A antibody H3FA antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST1H3A antibody histone H3/j antibody histone H3/i |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
Application
Histone H3 (tri-Methyl Lys9) antibody detects Histone H3 (tri-Methyl Lys9) protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys9) antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 μg of Histone H3 (tri-Methyl Lys9) . Western blot analysis was performed using Histone H3 (tri-Methyl Lys9) . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.
Application
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
