Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.Application
HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody diluted at 1:500 . Blue: Hoechst 33342 staining.Application
Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis. Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min. Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody diluted at 1:500. Red: alpha Tubulin antibody diluted at 1:2500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]Application
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Histone H3 (phospho Ser10) antibody dilution: 1:500.Application
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 µg 4T1 whole lysate/extract (untreated) B. 30 µg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 µg U2OS whole cell lysate/extract (untreated) B. 30 µg U2OS whole cell lysate/extract (serum deprivation for 48hr) C. 30 µg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr 15% SDS-PAGE Histone H3 (phospho Ser10) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments. IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody 15 % SDS-PAGE The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract (untreated) B. 30 µg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Histone H3S10ph (phospho Ser10) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (phospho Ser10) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | p-Ser10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBIHC-WChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3/A antibody H3FA antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST1H3A antibody histone H3/j antibody histone H3/i |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.
Application
HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody diluted at 1:500 . Blue: Hoechst 33342 staining.
Application
Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis. Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min. Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody diluted at 1:500. Red: alpha Tubulin antibody diluted at 1:2500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Application
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Histone H3 (phospho Ser10) antibody dilution: 1:500.
Application
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 µg 4T1 whole lysate/extract (untreated) B. 30 µg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 µg U2OS whole cell lysate/extract (untreated) B. 30 µg U2OS whole cell lysate/extract (serum deprivation for 48hr) C. 30 µg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr 15% SDS-PAGE Histone H3 (phospho Ser10) antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments. IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody 15 % SDS-PAGE The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract (untreated) B. 30 µg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | U2OS (100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
