Application
Dotblot analysis of anti-Histone3 (di-methyl Arg2) antibody with peptide samples. Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg2) antibody at different dilution as indicated. A: Peptide samples of Histone3 (di-methyl Arg2) B: Peptide samples of Histone H3.1Application
Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone3 (di-methyl Arg2) antibody dilution: 1:1000| Product Name | Histone H3R2me2 (Asymmetric dimethyl Arg2) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (di-methyl Arg2) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | Asymmetric dimethyl Arg2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 1 antibody methyl Histone H3 antibody H3a antibody histone H3/d antibody histone H3/i antibody histone H3/h antibody histone H3/j antibody H3R2me2 antibody Di methyl Histone H3 antibody histone H3/c antibody member A antibody HIS |
|---|---|
| Target | Histone H3 |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Dotblot analysis of anti-Histone3 (di-methyl Arg2) antibody with peptide samples. Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg2) antibody at different dilution as indicated. A: Peptide samples of Histone3 (di-methyl Arg2) B: Peptide samples of Histone H3.1
Application
Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone3 (di-methyl Arg2) antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
