Application
Dot blot analysis of methylation-peptide or non methylation-peptide (50ng per dot) using Histone H4K20me2 (di-Methyl Lys20) antibody at a concentration of 0.5ug/ml.Application
FACS analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using Histone H4K20me2 (di-Methyl Lys20) antibodyApplication
WB analysis of K562 cell lysate using Histone H4K20me2 (di-Methyl Lys20) antibody at a dilution of 1:1000. Lysate at 35ug per lane.| Product Name | Histone H4K20me2 (di-Methyl Lys20) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to H4K20me2Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008] |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H4. |
| Modification | di-Methyl Lys20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotFACSIHC-PWB |
| WB : 1:1000,DB |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIST2H4A HIST2H4 FO108 H4FN H4 H4F2 histone cluster 2 H4a H4/n |
|---|---|
| Target | HIST1H4A |
Application
Dot blot analysis of methylation-peptide or non methylation-peptide (50ng per dot) using Histone H4K20me2 (di-Methyl Lys20) antibody at a concentration of 0.5ug/ml.
Application
FACS analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using Histone H4K20me2 (di-Methyl Lys20) antibody
Application
WB analysis of K562 cell lysate using Histone H4K20me2 (di-Methyl Lys20) antibody at a dilution of 1:1000. Lysate at 35ug per lane.| Application Notes | WB : 1:1000,DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
