Application
ChIP was performed with NB4 nuclear extract and either 1 μg of H4K5,8,12ac antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to 1 kb upstream of the GAPDH gene or Sat2.Application
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K5acK8acK12ac antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ICC/IF analysis of HeLa cells using H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with the H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
ICC/IF analysis of HeLa cells using H4K5K8K12 unmodified peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5K8K12 unmodified peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
ICC/IF analysis of HeLa cells using H4K5acK8acK12ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5acK8acK12ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
ICC/IF analysis of HeLa cells using H2A.ZK5acK7acK11ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H2A.ZK5acK7acK11ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
ICC/IF analysis of HeLa cells using H4K5acK8acK12acK16ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5acK8acK12acK16ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
Dot blot analysis of peptides containing other histone modifications and the unmodified H4 using H4K5acK8acK12ac antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
ELISA was performed using a serial dilution of Histone H4K5acK8acK12ac (acetyl Lys5, Lys8, Lys12) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.| Product Name | Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) |
| Modification | acetyl Lys5/Lys8/Lys12 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFWBChIP |
| ChIP/ChIP-seq (0.5-1 μg/IP), ELISA (1:1000), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H4 acetyl Lys5 Lys8 Lys12 acetyl K5 K8 K12 Histone H4 H4FA acetyl Histone H4 histone cluster 1 H4a HIST1H4A Histone H4 acetyl K5 K8 K12 acetyl Lys5 Lys8 Lys12 Histone H4 Histone H4 acetyl |
|---|---|
| Target | Histone H4 (acetyl Lys5, Lys8, Lys12) |
Application
ChIP was performed with NB4 nuclear extract and either 1 μg of H4K5,8,12ac antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to 1 kb upstream of the GAPDH gene or Sat2.
Application
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K5acK8acK12ac antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ICC/IF analysis of HeLa cells using H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with the H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
ICC/IF analysis of HeLa cells using H4K5K8K12 unmodified peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5K8K12 unmodified peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
ICC/IF analysis of HeLa cells using H4K5acK8acK12ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5acK8acK12ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
ICC/IF analysis of HeLa cells using H2A.ZK5acK7acK11ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H2A.ZK5acK7acK11ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
ICC/IF analysis of HeLa cells using H4K5acK8acK12acK16ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5acK8acK12acK16ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
Dot blot analysis of peptides containing other histone modifications and the unmodified H4 using H4K5acK8acK12ac antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
ELISA was performed using a serial dilution of Histone H4K5acK8acK12ac (acetyl Lys5, Lys8, Lys12) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.| Application Notes | ChIP/ChIP-seq (0.5-1 μg/IP), ELISA (1:1000), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
