Application
Dot-blot analysis of all sorts of methylation peptides using Histone H4R3me1 (mono-methyl Arg3) antibody.Application
WB analysis of HeLa lysate and H4 protein using Histone H4R3me1 (mono-methyl Arg3) antibody.Application
ICC/IF analysis of 293T cells using Histone H4R3me1 (mono-methyl Arg3) antibody (green) and DAPI (blue).| Product Name | Histone H4R3me1 (mono-methyl Arg3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H4R3me1 (mono-methyl Arg3)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is mono-methylated. |
| Modification | mono-methyl Arg3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIPWB |
| WB: 1:500 - 1:200. IF: 1:50 - 1:20. IP: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H4F2 histone cluster 2 H4a H4 H4FN HIST2H4A HIST2H4 H4/n FO108 |
|---|---|
| Target | Histone H4R3me1 (mono-methyl Arg3) |
| Molecular Weight(MW) | 11 kDa (note) |
Application
Dot-blot analysis of all sorts of methylation peptides using Histone H4R3me1 (mono-methyl Arg3) antibody.
Application
WB analysis of HeLa lysate and H4 protein using Histone H4R3me1 (mono-methyl Arg3) antibody.
Application
ICC/IF analysis of 293T cells using Histone H4R3me1 (mono-methyl Arg3) antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:200. IF: 1:50 - 1:20. IP: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
